Conjugation

Part:BBa_K543032:Design

Designed by: Satoru Shimura   Group: iGEM11_Tokyo_Metropolitan   (2011-10-05)


assay system of killing by conjugation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 321
    Illegal NheI site found at 367
    Illegal NheI site found at 1028
    Illegal NheI site found at 1074
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1230
    Illegal NgoMIV site found at 1240
    Illegal NgoMIV site found at 2492
    Illegal NgoMIV site found at 2502
    Illegal AgeI site found at 3327
    Illegal AgeI site found at 3439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 404
    Illegal BsaI.rc site found at 2154
    Illegal SapI site found at 1132
    Illegal SapI site found at 2394


Design Notes

None


Source

BBa_J23100 BBa_I714051 BBa_J01003 BBa_I13504 BBa_J01003 BBa_I13507

References